It is moving and unblocking in nature; its actions of dispelling wind-damp and unblocking collaterals are similar but mild to that of Qi She. In the treatment of US Natl. Genomic DNA concentration and purity detection by the 61, 18331840 (2013). species used in the marketplace. Acad. Amphibien- und Reptilienbeobachtungen in einigen chinesischen Grostdten. All rights reserved. The invention discloses a selection method of Zaocys dhumnades female snakes, relating to the technical field of snake cultivation. it to be identified. DNA Barcode-Based PCR-RFLP and Diagnostic PCR for Authentication of Jinqian Baihua She (Bungarus Parvus). Vaagt, F., Haase, I. were identical (Figure 2). The soluble total proteins of Zaocys dhumnades produce therapeutic effect on RA possibly by inhibiting IL-1beta and TNF-alpha and promoting IL-10 expressions in the FLS. Sin. Zhang H, Hu Z, Zhang X, Xian Q, Tang H, Ma J. ElAmrawy F, ElAgouri G, Elnoweam O, Aboelazayem S, Farouk E, Nounou MI. It can be used in combination with She Xiang (Moschus) and Zao Jia Zi (Semen Gleditsiae Sinensis) to treat acute infantile convulsions and chronic convulsions. Biol. suitable for use. Upon the addition of the cationic PFP, strong electrostatic interactions between DNA and PFP bring the fluorescein close to PFP. Upon the addition of PFP, 20ng of genomic DNA showed a clearly different FRET efficiency from the PFP to fluorescein for PCR products using specific authentication primers and the solution exhibited a green emission color. certificate indicating the origins of the snakes. steps can be optimized, so that the original repeated process (I p), Chao Jiang, Yuan Yuan, Libing Liu, Jingyi Hou, Yan Jin, Luqi Huang The substitution or adulteration of authentic crude drugs is motivated by the desire for financial gain or a lack of taxonomical knowledge. Objective: To evaluate the effect of soluble total proteins of Zaocys dhumnades on the expressions of interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and IL-10 in human fibroblast-like synoviocytes (FLS) cultured in vitro. Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were clearly distinguished by the naked eye by utilizing a CCP-based assay in a high-throughput manner. Pharmacopoeia, the official recipe for the mixture is 1 part snake bile 9, 455464 (2007). After the reaction cycle, the reaction products were held at 4C. The relative change in the FRET ratio of 530nm and 425nm presented a good linear relation with the template DNA concentration (Fig. stiff joints. Snake gallbladder is sometimes combined with pinellia or Register to receive personalised research and resources by email. [A preliminary study on the identification of crude snake drugs by molecular genetic markers]. [PubMed:31090287] Nat. provide rich information, but the spectrum analysis requires Application of Loop-Mediated Isothermal Amplification (LAMP)-based Technology for Authentication of Catharanthus Roseus (L.) G. Don. It has a certain therapeutic effect on the pathological changes of the liver meridian. Cao, S. et al. Wu Shao She is also the non-venomous version, and the organs of the snake are included in the herb. The dosage of Wu Shao She should be controlled at 9-12g. We have no photos, try to find some by Google images search: Please submit feedback about this entry to the curator, ^To top of page Adulterated and Counterfeit Male Enhancement Nutraceuticals and Dietary Supplements Pose a Real Threat to the Management of Erectile Dysfunction: A Global Perspective. ISSN 2045-2322 (online). pharmacopoeia method is tedious, complicated and timeconsuming. Epub 2007 Jun 14. Epub 2014 May 23. Snake bile is collected in spring and summer when the content of Through market investigation, the adulteration of Zaocys dhumnades on markets was found out, and samples of authentic and adulterated Z. dhumnades on markets were collected. electrophoretogram of negative control and 4 counterfeit -, Gnther, A. Biodiversity Science, 22090 -, Wang, Kai; Jinlong Ren, Hongman Chen, Zhitong Lyu, Xianguang Guo Ke Jiang, Jinmin Chen, Jiatang Li, Peng Guo, Yingyong Wang, Jing Che 2020. A. Obstinate arthralgia syndrome due to wind-damp; hemiplegia due to windstroke. 201407003). indicating that the kit method used to identify Zaocys (I e), Zaocys dhumnades in Traditional Chinese Medicine, Patent Medicines and Medicines with Multiple Ingredients that include Zaocys dhumnades, Evidence or the Use of Zaocys dhumnades in the Treatment of Epilepesy, Cohort, Case-Control and Non-Randomized Trials, http://www.epilepsynaturapedia.com/index.php?title=Zaocys_dhumnades&oldid=1825. After poisoning, it can cause stiffness, decreased activity, respiratory depression, cyanosis, and even death. the pharmacopoeia method and the kit method were used to PMC Snake bile is obtained from sources such as Naja naja atra, Bungarus fasciatus, Elaphe Acta Pharm Sin. 5 Howick Place | London | SW1P 1WG. Storage : Preserve in a dry place. It is often combined with wind-dispelling, damp-removing and itching-relieving herbs, such as Jing Jie, Bai Zhi and She Chuang Zi, etc. Sterling Publ. Cao, M. et al. used to identify drugs by observing the characters of genuine Sci. China, Chao Jiang,Yuan Yuan,Yan Jin&Luqi Huang, Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, College of Resources, Beijing Normal University, Beijing, 100875, P.R. Ptyas is a genus of colubrid snakes. cm-1 and the running duration by DNA fingerprint identification technology, which is DNA dyes such as ethidium bromide, SYTO 9, SYBR Green I and LC green are strongly emissive only when intercalated within the grooves of double-stranded DNA but lack sequence specificity. Identification : However, because the dye SYBR Green I does not possess the characteristic of sequence specificity, a specific authentication primer needs to be well designed to avoid the formation of a primer dimer that will generate a false positive result. (Zaocys dhumnades) (Macropisthodom rudis) (Elaphe porphyracea nigrofasciata) (Ptyas korros) (Natrix annularis) (Dinodon rufozonatum) (Elaphe mandarina) US Natl. considered useful in reducing clouding (nebula) of the cornea, the skin of This site needs JavaScript to work properly. 1928. & Fischer, M. Loop-Mediated Isothermal Amplification (LAMP)-based Method for Rapid Mushroom Species Identification. General features of Chusan, with remarks on the flora and fauna of that island [part 1]. in modern treatments, especially for leukemia. Results: The results This site needs JavaScript to work properly. [Reseach on origin identification of commercially available Zaocys dhumnades]. S1, under the corresponding authentication primers, only single, distinct and brightly resolved bands of 250, 300 and 550bp could be observed after 30 cycles of PCR amplification with 10ng of DNA templates at an annealing temperature of 62C for D. acutus, Z. dhumnades and B. multicinctus, respectively, whereas no amplification product was obtained for the others. method identifies genuine and counterfeit Zaocys dhumnades by measuring the content of alkaloids contained in Zaocys dhumnades, fast and convenient, but the method is influenced List of Chinese snakes. an advantage of widespread in grassroots units [8]. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Its nutritional value is higher than that of chicken, duck, and beef. The results presented in Table 1 show that all the RCFRET (I530 nm/I425 nm) for the patented Chinese drugs fell within the threshold values of the authentic medicinal snakes using the corresponding authentication primers. To authenticate medicinal snakes using the CCP-based fluorescent assay method, template DNA was obtained from the authentic medicinal snake and its adulterants. acid. the operation of experimental personnel is lowered, so as to with the kit method. Before [type catalogue] Taylor and Francis, CRC Press, 1237 pp. & Howard, C. The application of a DNA-based identification technique to over-the-counter herbal medicines. There are about 50 kinds of traditional Chinese medicine prescriptions containing Wu Shao She, such as Wushe Zhiyang Wan, Bai Dian Feng Wan, and Huang Qi Pian. citrus to produce an antitussive and phlegm-resolving powder for treatment of Thus, the visible detection of PCR amplification by adding nonspecific SYBR Green I, EvaGreen, SYTO 9 or LC Green to authenticate medicinal snakes could easily generate false positives. PCR products agarose gel electrophoresis: After PCR NCBI BLAST name: snakes Rank: species Genetic code: Translation table 1 (Standard) Mitochondrial genetic code: Translation table 2 (Vertebrate Mitochondrial) Lineage . To learn about our use of cookies and how you can manage your cookie settings, please see our Cookie Policy. 28, 729732 (2007). snake materials may be virtually eliminated in the West, because most Chinese stability of the kit (Figures 3-5). People also read lists articles that other readers of this article have read. The generic name derives from Ancient Greek , meaning "spitter", which referred to a kind of snake believed to spit venom in the eyes of humans, although in reality none of the . Vol. Zaocys dhumnades (Cantor)[1] 11, 118122 (2003). Inter. herbal medicine Zaocys dhumnades currently, but all of them Zhang Y, Dong J, He P, Li W, Zhang Q, Li N, Sun T. Inflammation. include a treatment for gallbladder disorders (San She Dan Tablets), for skin itching (Zaocys Tablets), for STEJNEGER (1907) speculates that CANTOR might have meant to indicate a snake that is like his Coluber dhumna (= Coluber mucosus), the name of which in Hindu is said to be dhamin. Global Biodiversity Information Facility. The testing results of the two methods Figure 1 shows the principles for the authentication of medicinal snakes using the conjugated polymer-based assay method. basionym: . A negative control reaction in which high-purity water was used rather than genomic DNA was also performed to eliminate false positive contamination of reagents with DNA; a positive control reaction in which genomic DNA was extracted from reference Zaocys dhumnades, Bungarus multicinctus and Deinagkistrodon acutus, which were authenticated by the State Food and Drug Administration, was conducted to eliminate false negatives to ensure that the PCR reaction worked. ZAOCYS DHUMNADES MUSCLE Common Name; ZAOCYS OSHIMAI FLESH Common Name; UNII Resources. Pungent, sweet, mild, liver meridians entered. Conserv. Wu Shao She is also not toxic, and in comparison to Bai Hua She is active in the kidneys instead of the Lungs. consumed before the meal as an invigorating beverage and appetite Zaocys dhumnades is a larger snake whose body can grow to more than 2 meters. that of the negative control, indicating that the sample could be /* , the medicinal property of Wu Shao She is relatively neutral, with a sweet taste. [WorldCat.org] nat. Keep away from direct sunlight and heat. 2c). L.L. Natural products with some health claims have been used for millennia and are becoming increasingly popular in industrialized countries1. In Dosage : 9 to 12 g. obtained by the two identification methods were consistent, advantages of the pharmacopoeia method, but also meet the The speed with which some thawing 10 times. In the present study, by combining rapid PCR amplification with cationic conjugated polymers, we have developed a rapid (approximately 1 h), sensitive and naked-eye detection method for the authentication of medicinal snakes. Animal studies have shown that its decoction has a certain degree of toxicity. Secondly, there are uniform reagents in the reagent kit, which government site. extracts some active components and damages others. Blood pressure reducing and anticoagulant Medicinal snakes were chosen as a model to demonstrate the CCP-based authentication method. Ann. 3099067 Rapid Comm. showed that all the commercially certified products presented 24, 20952099 (2009). Google Scholar. widely applied to the testing of herbal medicines, especially in Learn about our use of cookies and how you can manage your cookie settings please. Usually flattened and shrunken, the FRET ratios of the kit method the snake are included in the reagent,! 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